Autoradiography is an ex-vivo imaging technique that measures the biodistribution of radiolabeled molecules at the cellular and tissue level. The technique requires frozen tissue sectioning (1-60 μm) using a cryostat, and imaging is obtained either through a film or a phosphor screen. Longitudinal sectioning and subsequent imaging of a whole animal is also possible with this technique.

The Center for Molecular Imaging currently houses a Leica CM3600 Whole-Animal Cryostat and a Phosphor imager (BAS 5000, Fujifilm) capable of sub-50 μm resolution.